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Evident Corporation simple pci imaging software
Simple Pci Imaging Software, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simple+pci+imaging+software/simple+pci+imaging+software/pmc03107777-63-17-21
Average 90 stars, based on 1 article reviews
simple pci imaging software - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

Imaging:

Article Title: Transformation of Sexually Transmitted Infection-Causing Serovars of Chlamydia trachomatis Using Blasticidin for Selection
Article Snippet: An IX-81 inverted fluorescence microscope (Olympus, Center Valley, PA) was used to visualize live cells without or with infection by serovar D wild type, plasmid-free CTD1.31 or CTD1-pGFPBSD transformant organisms. .. Simple PCI imaging software (Olympus) was used to acquire both bright field and green fluorescence images and Adobe Photoshop (Adobe, San Jose, CA) was used to do post-acquisition processing of the images. .. For immunofluorescence images, HeLa cells with or without chlamydial infection were fixed at 48 hours post infection with 2% paraformaldehyde dissolved in PBS for 30 min at room temperature, followed by permeabilization with 2% saponin (Sigma, St. Louis, MO) for an additional 30min.

Article Title: Methods and compositions for attenuated chlamydia as vaccine and vector
Article Snippet: A rabbit anti-chlamydial organism antibody, raised by immunization with purified C. muridarum elementary bodies, plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc., West Grove, Pa.) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus). ..

Article Title: Neutralizing antichlamydial activity of complement by chlamydia-secreted protease CPAF.
Article Snippet: Received 27 November 2015; accepted 5 July 2016 Available online ▪ ▪ ▪ 84 85 86 87 Abstract 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 Ascending infection by sexually transmitted Chlamydia trachomatis is required for chlamydial induction of tubal pathology.. To achieve ascension, the C. trachomatis organisms may have to spread from cell to cell, which inevitably exposes the organisms to extracellular mucosal effectors such as complement factors that are known to possess strong antichlamydial activities.. Here, we report that the chlamydia-secreted protease CPAF efficiently neutralized complement factor C3-dependent antichlamydial activity.

Article Title: Intrauterine Infection with Plasmid-Free Chlamydia muridarum Reveals a Critical Role of the Plasmid in Chlamydial Ascension and Establishes a Model for Evaluating Plasmid-Independent Pathogenicity
Article Snippet: A rabbit anti-chlamydial organism antibody plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green) (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus) as described previously (24). ..

Article Title: In Vitro Passage Selects for Chlamydia muridarum with Enhanced Infectivity in Cultured Cells but Attenuated Pathogenicity in Mouse Upper Genital Tract
Article Snippet: A rabbit antichlamydial antibody, raised by immunization with purified C. muridarum elementary bodies, plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired by using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus), as described previously ( 48 ). ..

Article Title: The chlamydial periplasmic stress response serine protease cHtrA is secreted into host cell cytosol
Article Snippet: .. The immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus, Melville, NY) as described previously [ ]. .. An Olympus FluoView laser confocal microscope (Olympus, Center Valley, PA) was used to further analyze some of the immunofluorescence samples at the University of Texas Health Science Center at San Antonio institutional core facility as described previously [ ].

Article Title: Chlamydia muridarum Induction of Glandular Duct Dilation in Mice
Article Snippet: .. The immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus) as described previously ( 37 ), while the H&E images were acquired under visible light. ..

Software:

Article Title: Transformation of Sexually Transmitted Infection-Causing Serovars of Chlamydia trachomatis Using Blasticidin for Selection
Article Snippet: An IX-81 inverted fluorescence microscope (Olympus, Center Valley, PA) was used to visualize live cells without or with infection by serovar D wild type, plasmid-free CTD1.31 or CTD1-pGFPBSD transformant organisms. .. Simple PCI imaging software (Olympus) was used to acquire both bright field and green fluorescence images and Adobe Photoshop (Adobe, San Jose, CA) was used to do post-acquisition processing of the images. .. For immunofluorescence images, HeLa cells with or without chlamydial infection were fixed at 48 hours post infection with 2% paraformaldehyde dissolved in PBS for 30 min at room temperature, followed by permeabilization with 2% saponin (Sigma, St. Louis, MO) for an additional 30min.

Article Title: Methods and compositions for attenuated chlamydia as vaccine and vector
Article Snippet: A rabbit anti-chlamydial organism antibody, raised by immunization with purified C. muridarum elementary bodies, plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc., West Grove, Pa.) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus). ..

Article Title: Neutralizing antichlamydial activity of complement by chlamydia-secreted protease CPAF.
Article Snippet: Received 27 November 2015; accepted 5 July 2016 Available online ▪ ▪ ▪ 84 85 86 87 Abstract 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 Ascending infection by sexually transmitted Chlamydia trachomatis is required for chlamydial induction of tubal pathology.. To achieve ascension, the C. trachomatis organisms may have to spread from cell to cell, which inevitably exposes the organisms to extracellular mucosal effectors such as complement factors that are known to possess strong antichlamydial activities.. Here, we report that the chlamydia-secreted protease CPAF efficiently neutralized complement factor C3-dependent antichlamydial activity.

Article Title: Intrauterine Infection with Plasmid-Free Chlamydia muridarum Reveals a Critical Role of the Plasmid in Chlamydial Ascension and Establishes a Model for Evaluating Plasmid-Independent Pathogenicity
Article Snippet: A rabbit anti-chlamydial organism antibody plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green) (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus) as described previously (24). ..

Article Title: In Vitro Passage Selects for Chlamydia muridarum with Enhanced Infectivity in Cultured Cells but Attenuated Pathogenicity in Mouse Upper Genital Tract
Article Snippet: A rabbit antichlamydial antibody, raised by immunization with purified C. muridarum elementary bodies, plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired by using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus), as described previously ( 48 ). ..

Article Title: The chlamydial periplasmic stress response serine protease cHtrA is secreted into host cell cytosol
Article Snippet: .. The immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus, Melville, NY) as described previously [ ]. .. An Olympus FluoView laser confocal microscope (Olympus, Center Valley, PA) was used to further analyze some of the immunofluorescence samples at the University of Texas Health Science Center at San Antonio institutional core facility as described previously [ ].

Article Title: Chlamydia muridarum Induction of Glandular Duct Dilation in Mice
Article Snippet: .. The immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus) as described previously ( 37 ), while the H&E images were acquired under visible light. ..

Fluorescence:

Article Title: Transformation of Sexually Transmitted Infection-Causing Serovars of Chlamydia trachomatis Using Blasticidin for Selection
Article Snippet: An IX-81 inverted fluorescence microscope (Olympus, Center Valley, PA) was used to visualize live cells without or with infection by serovar D wild type, plasmid-free CTD1.31 or CTD1-pGFPBSD transformant organisms. .. Simple PCI imaging software (Olympus) was used to acquire both bright field and green fluorescence images and Adobe Photoshop (Adobe, San Jose, CA) was used to do post-acquisition processing of the images. .. For immunofluorescence images, HeLa cells with or without chlamydial infection were fixed at 48 hours post infection with 2% paraformaldehyde dissolved in PBS for 30 min at room temperature, followed by permeabilization with 2% saponin (Sigma, St. Louis, MO) for an additional 30min.

Article Title: Methods and compositions for attenuated chlamydia as vaccine and vector
Article Snippet: A rabbit anti-chlamydial organism antibody, raised by immunization with purified C. muridarum elementary bodies, plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc., West Grove, Pa.) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus). ..

Article Title: Neutralizing antichlamydial activity of complement by chlamydia-secreted protease CPAF.
Article Snippet: Received 27 November 2015; accepted 5 July 2016 Available online ▪ ▪ ▪ 84 85 86 87 Abstract 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 Ascending infection by sexually transmitted Chlamydia trachomatis is required for chlamydial induction of tubal pathology.. To achieve ascension, the C. trachomatis organisms may have to spread from cell to cell, which inevitably exposes the organisms to extracellular mucosal effectors such as complement factors that are known to possess strong antichlamydial activities.. Here, we report that the chlamydia-secreted protease CPAF efficiently neutralized complement factor C3-dependent antichlamydial activity.

Article Title: Intrauterine Infection with Plasmid-Free Chlamydia muridarum Reveals a Critical Role of the Plasmid in Chlamydial Ascension and Establishes a Model for Evaluating Plasmid-Independent Pathogenicity
Article Snippet: A rabbit anti-chlamydial organism antibody plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green) (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus) as described previously (24). ..

Article Title: In Vitro Passage Selects for Chlamydia muridarum with Enhanced Infectivity in Cultured Cells but Attenuated Pathogenicity in Mouse Upper Genital Tract
Article Snippet: A rabbit antichlamydial antibody, raised by immunization with purified C. muridarum elementary bodies, plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired by using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus), as described previously ( 48 ). ..

Article Title: The chlamydial periplasmic stress response serine protease cHtrA is secreted into host cell cytosol
Article Snippet: .. The immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus, Melville, NY) as described previously [ ]. .. An Olympus FluoView laser confocal microscope (Olympus, Center Valley, PA) was used to further analyze some of the immunofluorescence samples at the University of Texas Health Science Center at San Antonio institutional core facility as described previously [ ].

Article Title: Chlamydia muridarum Induction of Glandular Duct Dilation in Mice
Article Snippet: .. The immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus) as described previously ( 37 ), while the H&E images were acquired under visible light. ..

Immunofluorescence:

Article Title: Methods and compositions for attenuated chlamydia as vaccine and vector
Article Snippet: A rabbit anti-chlamydial organism antibody, raised by immunization with purified C. muridarum elementary bodies, plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc., West Grove, Pa.) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus). ..

Article Title: Neutralizing antichlamydial activity of complement by chlamydia-secreted protease CPAF.
Article Snippet: Received 27 November 2015; accepted 5 July 2016 Available online ▪ ▪ ▪ 84 85 86 87 Abstract 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 Ascending infection by sexually transmitted Chlamydia trachomatis is required for chlamydial induction of tubal pathology.. To achieve ascension, the C. trachomatis organisms may have to spread from cell to cell, which inevitably exposes the organisms to extracellular mucosal effectors such as complement factors that are known to possess strong antichlamydial activities.. Here, we report that the chlamydia-secreted protease CPAF efficiently neutralized complement factor C3-dependent antichlamydial activity.

Article Title: Intrauterine Infection with Plasmid-Free Chlamydia muridarum Reveals a Critical Role of the Plasmid in Chlamydial Ascension and Establishes a Model for Evaluating Plasmid-Independent Pathogenicity
Article Snippet: A rabbit anti-chlamydial organism antibody plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green) (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus) as described previously (24). ..

Article Title: In Vitro Passage Selects for Chlamydia muridarum with Enhanced Infectivity in Cultured Cells but Attenuated Pathogenicity in Mouse Upper Genital Tract
Article Snippet: A rabbit antichlamydial antibody, raised by immunization with purified C. muridarum elementary bodies, plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired by using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus), as described previously ( 48 ). ..

Article Title: The chlamydial periplasmic stress response serine protease cHtrA is secreted into host cell cytosol
Article Snippet: .. The immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus, Melville, NY) as described previously [ ]. .. An Olympus FluoView laser confocal microscope (Olympus, Center Valley, PA) was used to further analyze some of the immunofluorescence samples at the University of Texas Health Science Center at San Antonio institutional core facility as described previously [ ].

Article Title: Chlamydia muridarum Induction of Glandular Duct Dilation in Mice
Article Snippet: .. The immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus) as described previously ( 37 ), while the H&E images were acquired under visible light. ..

Microscopy:

Article Title: Methods and compositions for attenuated chlamydia as vaccine and vector
Article Snippet: A rabbit anti-chlamydial organism antibody, raised by immunization with purified C. muridarum elementary bodies, plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc., West Grove, Pa.) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus). ..

Article Title: Neutralizing antichlamydial activity of complement by chlamydia-secreted protease CPAF.
Article Snippet: Received 27 November 2015; accepted 5 July 2016 Available online ▪ ▪ ▪ 84 85 86 87 Abstract 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 Ascending infection by sexually transmitted Chlamydia trachomatis is required for chlamydial induction of tubal pathology.. To achieve ascension, the C. trachomatis organisms may have to spread from cell to cell, which inevitably exposes the organisms to extracellular mucosal effectors such as complement factors that are known to possess strong antichlamydial activities.. Here, we report that the chlamydia-secreted protease CPAF efficiently neutralized complement factor C3-dependent antichlamydial activity.

Article Title: Intrauterine Infection with Plasmid-Free Chlamydia muridarum Reveals a Critical Role of the Plasmid in Chlamydial Ascension and Establishes a Model for Evaluating Plasmid-Independent Pathogenicity
Article Snippet: A rabbit anti-chlamydial organism antibody plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green) (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus) as described previously (24). ..

Article Title: In Vitro Passage Selects for Chlamydia muridarum with Enhanced Infectivity in Cultured Cells but Attenuated Pathogenicity in Mouse Upper Genital Tract
Article Snippet: A rabbit antichlamydial antibody, raised by immunization with purified C. muridarum elementary bodies, plus a goat anti-rabbit IgG secondary antibody conjugated with Cy2 (green; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial organism-containing inclusions. .. Immunofluorescence images were acquired by using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus), as described previously ( 48 ). ..

Article Title: The chlamydial periplasmic stress response serine protease cHtrA is secreted into host cell cytosol
Article Snippet: .. The immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus, Melville, NY) as described previously [ ]. .. An Olympus FluoView laser confocal microscope (Olympus, Center Valley, PA) was used to further analyze some of the immunofluorescence samples at the University of Texas Health Science Center at San Antonio institutional core facility as described previously [ ].

Article Title: Chlamydia muridarum Induction of Glandular Duct Dilation in Mice
Article Snippet: .. The immunofluorescence images were acquired using an Olympus AX-70 fluorescence microscope equipped with multiple filter sets and Simple PCI imaging software (Olympus) as described previously ( 37 ), while the H&E images were acquired under visible light. ..

other:

Article Title: Chlamydial Plasmid-Encoded Virulence Factor Pgp3 Neutralizes the Antichlamydial Activity of Human Cathelicidin LL-37
Article Snippet: HeLa cells grown on coverslips were fixed with 2% (vol/vol) paraformaldehyde (Sigma) dissolved in phosphate-buffered saline (PBS) for 30 min at room temperature, followed by permeabilization with 2% (wt/vol) saponin (Sigma) for an additional 30 min. After washing and blocking, the cell samples were subjected to antibody and chemical staining.



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Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple <t>PCI</t> image <t>analysis</t> <t>software</t> (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.
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Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple <t>PCI</t> image <t>analysis</t> <t>software</t> (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.
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Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple <t>PCI</t> image <t>analysis</t> <t>software</t> (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.
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Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple <t>PCI</t> image <t>analysis</t> <t>software</t> (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.
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Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple <t>PCI</t> image <t>analysis</t> <t>software</t> (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.
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Hamamatsu simple pci image analysis software
Semiquantitative assessment and localization of CBS and CSE. UA sections were labeled with antibodies against CBS, or CSE (not shown), followed by secondary Alexa488 (green)-labeled secondary antibody. Endothelial cells were labeled with CD31 followed by Alexa568 (red)-labeled secondary antibody. Cell nuclei were stained with DAPI. Negative control treated with IgG or with primary antibody omitted is shown in first panel. Representative outline of border between UA intima and media was indicated, and UA lumen (l), intima (i), and media (m) were denoted (second panel). Regions of interest for intima (ROI – i) and media (ROI – m) used for analysis in Simple <t>PCI</t> <t>software</t> are illustrated (third panel). Scale bar is 25 μm
Simple Pci Image Analysis Software, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple PCI image analysis software (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.

Journal: Fertility and sterility

Article Title: Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3′,5′-cyclic monophosphate signaling

doi: 10.1016/j.fertnstert.2015.02.025

Figure Lengend Snippet: Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple PCI image analysis software (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.

Article Snippet: Images were processed to determine gray level using Simple PCI (Hamamatsu) imaging software, as previously described ( 31 , 32 ).

Techniques: Cell Culture, Labeling, Software

Semiquantitative assessment and localization of CBS and CSE. UA sections were labeled with antibodies against CBS, or CSE (not shown), followed by secondary Alexa488 (green)-labeled secondary antibody. Endothelial cells were labeled with CD31 followed by Alexa568 (red)-labeled secondary antibody. Cell nuclei were stained with DAPI. Negative control treated with IgG or with primary antibody omitted is shown in first panel. Representative outline of border between UA intima and media was indicated, and UA lumen (l), intima (i), and media (m) were denoted (second panel). Regions of interest for intima (ROI – i) and media (ROI – m) used for analysis in Simple PCI software are illustrated (third panel). Scale bar is 25 μm

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Analysis of Vascular Hydrogen Sulfide Biosynthesis

doi: 10.1007/978-1-4939-9528-8_3

Figure Lengend Snippet: Semiquantitative assessment and localization of CBS and CSE. UA sections were labeled with antibodies against CBS, or CSE (not shown), followed by secondary Alexa488 (green)-labeled secondary antibody. Endothelial cells were labeled with CD31 followed by Alexa568 (red)-labeled secondary antibody. Cell nuclei were stained with DAPI. Negative control treated with IgG or with primary antibody omitted is shown in first panel. Representative outline of border between UA intima and media was indicated, and UA lumen (l), intima (i), and media (m) were denoted (second panel). Regions of interest for intima (ROI – i) and media (ROI – m) used for analysis in Simple PCI software are illustrated (third panel). Scale bar is 25 μm

Article Snippet: Image Analysis list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Open image in Simple PCI Image analysis software (Hamamatsu Corp, Sewickley, PA, USA).

Techniques: Labeling, Staining, Negative Control, Software